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pBIG2ABC-eIF3Δbgi
(Plasmid #240043)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240043 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBIG2x
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Gentamicin, 25 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eIF3k, l, e, f, h, d, m, a, c
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • eIF3a: TEV_3xFLAG, eIF3c: 3C_STREP II, eIF3d: HA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBIG2ABC-eIF3Δbgi was a gift from Venki Ramakrishnan (Addgene plasmid # 240043 ; http://n2t.net/addgene:240043 ; RRID:Addgene_240043)

  • For your References section:

    Purification and characterization of recombinant human translation initiation factor eIF3. Diaz-Lopez I, Gordiyenko Y, Zuber PK, Li X, Ramakrishnan V. Protein Sci. 2026 Jan;35(1):e70388. doi: 10.1002/pro.70388. 10.1002/pro.70388 PubMed 41432235
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