pAAV-cjD2-E8-CMVmini-EGFP-miR-WPRE3-hGHpA
(Plasmid
#240049)
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PurposeExpression of GFP in striatal D2 medium spiny neurons in marmoset
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240049 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesSynthetic
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Insert Size (bp)717
- Promoter CMVmini
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ttaggcgtgtacggt (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. The Addgene sequence finds the removal of the AscI restriction site downstream of EGFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-cjD2-E8-CMVmini-EGFP-miR-WPRE3-hGHpA was a gift from Guoping Feng (Addgene plasmid # 240049 ; http://n2t.net/addgene:240049 ; RRID:Addgene_240049)