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Addgene

pMKR07
(Plasmid #240097)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240097 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pYH7
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology
  • Promoter ermE* promoter

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-6229288/v1 for the article preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMKR07 was a gift from Adrian Keatinge-Clay (Addgene plasmid # 240097 ; http://n2t.net/addgene:240097 ; RRID:Addgene_240097)

  • For your References section:

    A CRISPR-Cas9 System for Knock-out and Knock-in of High Molecular Weight DNA Enables Module-Swapping of the Pikromycin Synthase in its Native Host. Wang ZC, Stegall H, Miyazawa T, Keatinge-Clay AT. Res Sq [Preprint]. 2025 Mar 27:rs.3.rs-6229288. doi: 10.21203/rs.3.rs-6229288/v1. 10.21203/rs.3.rs-6229288/v1 PubMed 40195982
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