Skip to main content
Addgene
Open Menu

PAX3::FOXO1ΔHD-2A-sfGFP
(Plasmid #240099)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240099 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pCS2+MCS-P2A-sfGFP
  • Backbone manufacturer
    Jason Berman (Addgene Plasmid #74668)
  • Backbone size w/o insert (bp) 4914
  • Total vector size (bp) 7244
  • Modifications to backbone
    Add human PAX3::FOXO1-minusHD with upstream SP6 and Kozak sequence
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PAX3::FOXO1-minusHD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2353
  • Mutation
    removed amino acids 218-278 for the homeobox DNA binding domain
  • GenBank ID
  • Entrez Gene
    FOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
  • Entrez Gene
    PAX3 (a.k.a. CDHS, HUP2, PAX-3, WS1, WS3)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.12.603270 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PAX3::FOXO1ΔHD-2A-sfGFP was a gift from Genevieve Kendall (Addgene plasmid # 240099 ; http://n2t.net/addgene:240099 ; RRID:Addgene_240099)

  • For your References section:

    Rhabdomyosarcoma fusion oncoprotein initially pioneers a neural signature in vivo. Kucinski J, Tallan A, Taslim C, Vontell AM, Silvius KM, Wang M, Cannon MV, Stanton BZ, Kendall GC. Cell Rep. 2025 Jul 22;44(7):115923. doi: 10.1016/j.celrep.2025.115923. Epub 2025 Jul 3. 10.1016/j.celrep.2025.115923 PubMed 40614256
Related items: