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Addgene

pHR-PUM.HD-mCherry-Cry2WT
(Plasmid #240102)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240102 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSFFV
  • Vector type
    Mammalian Expression, Bacterial Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pumilio homology domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3900
  • Promoter SFFV promoter
  • Tag / Fusion Protein
    • mCherry and Cry2 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-PUM.HD-mCherry-Cry2WT was a gift from Liam Holt (Addgene plasmid # 240102 ; http://n2t.net/addgene:240102 ; RRID:Addgene_240102)

  • For your References section:

    Polysome collapse and RNA condensation fluidize the cytoplasm. Xie Y, Shu T, Liu T, Spindler MC, Mahamid J, Hocky GM, Gresham D, Holt LJ. Mol Cell. 2024 Jul 25;84(14):2698-2716.e9. doi: 10.1016/j.molcel.2024.06.024. 10.1016/j.molcel.2024.06.024 PubMed 39059370
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