TurboID-NPM1c-MSCV-IRES-GFP
(Plasmid
#240130)
-
PurposeExpresses TurboID-NPM1c fusion in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 240130 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backboneMSCV
- Backbone size w/o insert (bp) 6437
- Total vector size (bp) 8360
-
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNPM1c
-
Alt nameNPMc
-
SpeciesH. sapiens (human)
-
Insert Size (bp)894
-
MutationC-terminal frameshift mutation as seen in Acute Myeloid Leukemia
-
Entrez GeneNPM1 (a.k.a. B23, NPM)
-
Tags
/ Fusion Proteins
- TurboID (N terminal on insert)
- IRES-GFP (C terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
TurboID-NPM1c-MSCV-IRES-GFP was a gift from Timothy Ley (Addgene plasmid # 240130 ; http://n2t.net/addgene:240130 ; RRID:Addgene_240130) -
For your References section:
Overexpression of the signaling-coordinator GAB2 can play an important role in Acute Myeloid Leukemia progression. Kramer MH, Richardson SN, Li Y, Yin T, Helton NM, George DR, Cai M, Ramakrishnan SM, Katerndahl CD, Miller CA, Ley TJ. J Clin Invest. 2025 Aug 7:e195929. doi: 10.1172/JCI195929. 10.1172/JCI195929 PubMed 40773291