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pUHD 10-3-human CDK11 p110B D543→N mut. cat.-FLAG
(Plasmid #240139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUHD10-3
  • Backbone size w/o insert (bp) 3146
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDK11 p110
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2328
  • Mutation
    D543N
  • GenBank ID
    AF067520
  • Entrez Gene
    CDK11A (a.k.a. CDC2L2, CDC2L3, CDK11-p110, CDK11-p46, CDK11-p58, PITSLRE, p58GTA)
  • Promoter TRE, CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer min.CMV forw
  • 3′ sequencing primer SV40pA forw
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid developed at St Jude Children's Research Hospital by the late Drs Vincent Kidd and Jill Lahti. Dr Roussel is responsible for the distribution of the plasmids.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUHD 10-3-human CDK11 p110B D543→N mut. cat.-FLAG was a gift from Vincent Kidd & Jill Lahti & Martine Roussel (Addgene plasmid # 240139 ; http://n2t.net/addgene:240139 ; RRID:Addgene_240139)

  • For your References section:

    Characterization of cyclin L1 and L2 interactions with CDK11 and splicing factors: influence of cyclin L isoforms on splice site selection. Loyer P, Trembley JH, Grenet JA, Busson A, Corlu A, Zhao W, Kocak M, Kidd VJ, Lahti JM. J Biol Chem. 2008 Mar 21;283(12):7721-32. doi: 10.1074/jbc.M708188200. Epub 2008 Jan 23. 10.1074/jbc.M708188200 PubMed 18216018
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