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pFLEX-FLAG-human Cyclin L1 beta
(Plasmid #240147)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFLEX
  • Backbone size w/o insert (bp) 3442
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cyclin L1 beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    696
  • Entrez Gene
    CCNL1 (a.k.a. ANIA6A, BM-001, PRO1073, ania-6a)
  • Promoter SRalpha
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pFLEX forw
  • 3′ sequencing primer pFLEX rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid developed at St Jude Children's Research Hospital by the late Drs Vincent Kidd and Jill Lahti. Dr Roussel is responsible for the distribution of the plasmids.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLEX-FLAG-human Cyclin L1 beta was a gift from Vincent Kidd & Jill Lahti & Martine Roussel (Addgene plasmid # 240147 ; http://n2t.net/addgene:240147 ; RRID:Addgene_240147)

  • For your References section:

    Characterization of cyclin L1 and L2 interactions with CDK11 and splicing factors: influence of cyclin L isoforms on splice site selection. Loyer P, Trembley JH, Grenet JA, Busson A, Corlu A, Zhao W, Kocak M, Kidd VJ, Lahti JM. J Biol Chem. 2008 Mar 21;283(12):7721-32. doi: 10.1074/jbc.M708188200. Epub 2008 Jan 23. 10.1074/jbc.M708188200 PubMed 18216018
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