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His-lsMSP1D1dH5
(Plasmid #240150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240150 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5246
  • Total vector size (bp) 5885
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    His-lsMSP1D1dH5
  • Species
    Synthetic
  • Insert Size (bp)
    639
  • Promoter T7
  • Tags / Fusion Proteins
    • His-tag (N terminal on insert)
    • His-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GCGAAATTAATACGACTCACTATAGG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-lsMSP1D1dH5 was a gift from Lise Arleth (Addgene plasmid # 240150 ; http://n2t.net/addgene:240150 ; RRID:Addgene_240150)

  • For your References section:

    Non-ionic detergent assists formation of supercharged nanodiscs and insertion of membrane proteins. Tidemand FG, Blemmer S, Johansen NT, Arleth L, Pedersen MC. Biochim Biophys Acta Biomembr. 2022 Jun 1;1864(6):183884. doi: 10.1016/j.bbamem.2022.183884. Epub 2022 Feb 16. 10.1016/j.bbamem.2022.183884 PubMed 35182589
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