pRedCas9
(Plasmid
#240153)
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PurposeExpresses lambda red machinery and cas9
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240153 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepSC101
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namegam
Gene/Insert 2
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Gene/Insert namebeta
Gene/Insert 3
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Gene/Insert nameexo
Gene/Insert 4
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Gene/Insert namecas9
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.06.19.660532 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRedCas9 was a gift from Morten Norholm (Addgene plasmid # 240153 ; http://n2t.net/addgene:240153 ; RRID:Addgene_240153) -
For your References section:
Iterative in vivo cut’n’paste of chromosomal loci in Escherichia coli K-12 using synthetic DNA. Gude S, Bertelsen ABB, Nørholm MHH. bioRxiv 2025.06.19.660532 10.1101/2025.06.19.660532
