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pCut_GFP
(Plasmid #240154)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240154 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    ColA
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    constant cut sites CRISPR guide array

Gene/Insert 2

  • Gene/Insert name
    "self-curing" CRISPR guide array

Gene/Insert 3

  • Gene/Insert name
    GFP

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.19.660532 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCut_GFP was a gift from Morten Norholm (Addgene plasmid # 240154 ; http://n2t.net/addgene:240154 ; RRID:Addgene_240154)

  • For your References section:

    Iterative in vivo cut’n’paste of chromosomal loci in Escherichia coli K-12 using synthetic DNA. Gude S, Bertelsen ABB, Nørholm MHH. bioRxiv 2025.06.19.660532 10.1101/2025.06.19.660532
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