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Addgene

pAJC1
(Plasmid #240164)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240164 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET41 a(=)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5933
  • Total vector size (bp) 5925
  • Vector type
    Bacterial Expression
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    katPc
  • Alt name
    Pyrobaculum calidifontis manganese dependent catalase
  • Alt name
    manganese catalase
  • Alt name
    Pc-kat
  • Species
    Pyrobaculum calidifontis
  • Insert Size (bp)
    897
  • GenBank ID
    AB076020 ab076020
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer nanopore sequencing. no sequencing primer used.
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Pc- kat
  • Alt name
    Pyrobaculum calidifontis manganese catalase
  • Species
    Pyrobaculum calidifontis
  • Insert Size (bp)
    897
  • GenBank ID

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAJC1 was a gift from Danielle Saunders (Addgene plasmid # 240164 ; http://n2t.net/addgene:240164 ; RRID:Addgene_240164)
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