pAJC1
(Plasmid #240164)

• Purpose: Production of a thermostable enzyme, Pyrobaculum calidifontis manganese-dependent catalase, to be used in an educational laboratory exercise.
• Depositing Lab: Danielle Saunders
• Publication: Overproduction of a recombinant heat stable catalase for use in an extremophile enzyme activity lab. (unpublished)

Backbone

• Vector backbone: pET41 a(=)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5933
• Total vector size (bp): 5925
• Vector type: Bacterial Expression
• Selectable markers: Kanamycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: katPc
• Alt name: Pyrobaculum calidifontis manganese dependent catalase
• Alt name: manganese catalase
• Alt name: Pc-kat
• Species: Pyrobaculum calidifontis
• Insert Size (bp): 897
• GenBank ID: AB076020 ab076020
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: nanopore sequencing. no sequencing primer used.

Gene/Insert 2

• Gene/Insert name: Pc- kat
• Alt name: Pyrobaculum calidifontis manganese catalase
• Species: Pyrobaculum calidifontis
• Insert Size (bp): 897
• GenBank ID:

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry