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Addgene

pJM220-PhoA-mNeongreen-J23101-mScarletI
(Plasmid #240201)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240201 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pJM220
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 6690

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mNeongreen
  • Promoter PphoA

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    mScarlet-I
  • Promoter J23101

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJM220-PhoA-mNeongreen-J23101-mScarletI was a gift from Richard Murray (Addgene plasmid # 240201 ; http://n2t.net/addgene:240201 ; RRID:Addgene_240201)
  • For your References section:

    Engineering the Soil Bacterium Pseudomonas synxantha 2-79 into a Ratiometric Bioreporter for Phosphorus Limitation. Larsson EM, Murray RM, Newman DK. ACS Synth Biol. 2024 Jan 19;13(1):384-393. doi: 10.1021/acssynbio.3c00642. Epub 2024 Jan 2. 10.1021/acssynbio.3c00642 PubMed 38165130