pSC101-MP-mRFP1
(Plasmid
#240204)
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PurposeLow copy mRFP1 reporter for monitoring metabolite-responsive CRISPRa activity
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * | |
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Backbone
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Vector backbonepSC101
- Backbone size w/o insert (bp) 3753
- Total vector size (bp) 4431
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemRFP1
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SpeciesSynthetic
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Insert Size (bp)678
- Promoter J3-DA9-UP1-MP1
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer None (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byEngineered promoter sourced from Jesse Zalatan and James Carothers (Burbano, D.A. et al. PNAS, 120, 30, e2220358120, 2023).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSC101-MP-mRFP1 was a gift from Wilfred Chen (Addgene plasmid # 240204 ; http://n2t.net/addgene:240204 ; RRID:Addgene_240204) -
For your References section:
Metabolite-responsive scaffold RNAs for dynamic CRISPR transcriptional regulation. Stohr AM, Hansen H, Richards B, Park H, Goncalves AG, Agrawal A, Blenner M, Chen W. Nucleic Acids Res. 2025 Nov 13;53(21):gkaf1290. doi: 10.1093/nar/gkaf1290. 10.1093/nar/gkaf1290 PubMed 41277686