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pColE1-MP-Violacein
(Plasmid #240205)

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Item Catalog # Description Quantity Price (USD)
Plasmid 240205 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *
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Backbone

  • Vector backbone
    pColE1
  • Backbone size w/o insert (bp) 3075
  • Total vector size (bp) 10541
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    vioA-vioB-vioC-vioD-vioE
  • Species
    Synthetic
  • Insert Size (bp)
    7466
  • Promoter J3-DA9-UP1-MP1

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Engineered promoter sourced from Jesse Zalatan and James Carothers (Burbano, D.A. et al. PNAS, 120, 30, e2220358120, 2023). Violacein biosynthesis genes sourced from Mattheos Koffas (Addgene plasmid 73440 )

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pColE1-MP-Violacein was a gift from Wilfred Chen (Addgene plasmid # 240205 ; http://n2t.net/addgene:240205 ; RRID:Addgene_240205)

  • For your References section:

    Metabolite-responsive scaffold RNAs for dynamic CRISPR transcriptional regulation. Stohr AM, Hansen H, Richards B, Park H, Goncalves AG, Agrawal A, Blenner M, Chen W. Nucleic Acids Res. 2025 Nov 13;53(21):gkaf1290. doi: 10.1093/nar/gkaf1290. 10.1093/nar/gkaf1290 PubMed 41277686
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