pCRISPRa-Trp
(Plasmid #240209)

• Purpose: Bacterial CRISPRa vector expressing dCas9, MCP-SoxS, and a tryptophan-responsive MR-scRNA
• Depositing Lab: Wilfred Chen
• Publication: Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gkaf1290. doi: 10.1093/nar/gkaf1290.

Backbone

• Vector backbone: p15a
• Total vector size (bp): 8135
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: dCas9
• Species: Synthetic
• Insert Size (bp): 4107
• Promoter: Sp.pCas9

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: None

Gene/Insert 2

• Gene/Insert name: MCP-SoxS
• Species: Synthetic
• Insert Size (bp): 696
• Promoter: J23107

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: None

Gene/Insert 3

• Gene/Insert name: Tryptophan MR-scRNA
• Species: Synthetic
• Insert Size (bp): 166
• Promoter: J23119

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: None

Resource Information

• Supplemental Documents:
  • pcrispra-trp.gb
• A portion of this plasmid was derived from a plasmid made by: Jesse Zalatan (Addgene plasmid 153025)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry