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pSC-pGAL10-CRISPRa
(Plasmid #240210)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240210 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pColE1
  • Total vector size (bp) 15201
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    MCP-VPR
  • Species
    Synthetic
  • Promoter pGAL10

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    dCas9
  • Species
    Synthetic
  • Promoter pGAL10

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Wendell Lim and Stanley Qi (Addgene plasmid 62283)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSC-pGAL10-CRISPRa was a gift from Wilfred Chen (Addgene plasmid # 240210 ; http://n2t.net/addgene:240210 ; RRID:Addgene_240210)

  • For your References section:

    Metabolite-responsive scaffold RNAs for dynamic CRISPR transcriptional regulation. Stohr AM, Hansen H, Richards B, Park H, Goncalves AG, Agrawal A, Blenner M, Chen W. Nucleic Acids Res. 2025 Nov 13;53(21):gkaf1290. doi: 10.1093/nar/gkaf1290. 10.1093/nar/gkaf1290 PubMed 41277686
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