Skip to main content
Addgene
Open Menu

pHS-dCas9
(Plasmid #240212)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240212 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pUC
  • Backbone size w/o insert (bp) 4046
  • Total vector size (bp) 8276
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4230
  • Promoter PGK
  • Tag / Fusion Protein
    • NLS (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Stanley Qi (Addgene plasmid 84239)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS-dCas9 was a gift from Wilfred Chen (Addgene plasmid # 240212 ; http://n2t.net/addgene:240212 ; RRID:Addgene_240212)

  • For your References section:

    Metabolite-responsive scaffold RNAs for dynamic CRISPR transcriptional regulation. Stohr AM, Hansen H, Richards B, Park H, Goncalves AG, Agrawal A, Blenner M, Chen W. Nucleic Acids Res. 2025 Nov 13;53(21):gkaf1290. doi: 10.1093/nar/gkaf1290. 10.1093/nar/gkaf1290 PubMed 41277686
Related items: