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Addgene

pENTR_spatzle-HA
(Plasmid #240227)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240227 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEntr
  • Backbone manufacturer
    Thermo Fisher
  • Vector type
    Gateway shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    spatzle
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    spz (a.k.a. Dmel_CG6134, CG6134, CT19282, Dmel\CG6134, SPZ, Spatzle, Spz, Spz-1, Spz1, mel(3)7)
  • Tag / Fusion Protein
    • HA-spGFP11 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.07.09.659702 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR_spatzle-HA was a gift from Justin Bosch & Norbert Perrimon (Addgene plasmid # 240227 ; http://n2t.net/addgene:240227 ; RRID:Addgene_240227)

  • For your References section:

    Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking. Bosch JA, Beltran PMJ, Cavers C, LaGraff JT, Melanson R, Singh A, Chen W, Hu Y, Tattikota SG, Liu Y, Hashmi Y, Asara JM, Branon T, Ting AY, Carr SA, Perrimon N. bioRxiv 2025.07.09.659702; doi: https://doi.org/10.1101/2025.07.09.659702 10.1101/2025.07.09.659702
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