pMT-sfGFP-TurboID-ER
(Plasmid
#240230)
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PurposeExpression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240230 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMK33-GW
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Backbone manufacturerNorbert Perrimon Lab
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Vector typeInsect Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBIP-sfGFP-TurboID-KDEL
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Alt nameGFP-TurboID-ER
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SpeciesSynthetic
- Promoter MT
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.07.09.659702 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMT-sfGFP-TurboID-ER was a gift from Justin Bosch & Norbert Perrimon (Addgene plasmid # 240230 ; http://n2t.net/addgene:240230 ; RRID:Addgene_240230) -
For your References section:
Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking. Bosch JA, Beltran PMJ, Cavers C, LaGraff JT, Melanson R, Singh A, Chen W, Hu Y, Tattikota SG, Liu Y, Hashmi Y, Asara JM, Branon T, Ting AY, Carr SA, Perrimon N. bioRxiv 2025.07.09.659702; doi: https://doi.org/10.1101/2025.07.09.659702 10.1101/2025.07.09.659702