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R26_CAG_BST_FUCCIplex
(Plasmid #240241)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240241 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC plasmid
  • Backbone size w/o insert (bp) 7195
  • Total vector size (bp) 9613
  • Modifications to backbone
    Inserted left and right hROSA26 homology arms, blasticidin resistance gene trap (adenovirus splice acceptor, T2A, BlastR, bGH pA), CAG promoter and bGH polyA
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FUCCIplex
  • Species
    Synthetic
  • Insert Size (bp)
    2418
  • Promoter CAG

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    R26_CAG_BST_FUCCIplex was generated starting from the pR26-Bst_CAG-Tet-On-3G construct, which was a gift from Dr. Alessandro Bertero (https://doi.org/10.12688/openreseurope.19024.1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.12.19.629259 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R26_CAG_BST_FUCCIplex was a gift from Francesco Pasqualini (Addgene plasmid # 240241 ; http://n2t.net/addgene:240241 ; RRID:Addgene_240241)

  • For your References section:

    CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies. Di Sante M, Pezzotti M, Zimmermann J, Enrico A, Deschamps J, Balmas E, Becca S, Reali A, Bertero A, Jug F, Pasqualini FS. bioRxiv 2024.12.19.629259 10.1101/2024.12.19.629259
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