pET15b-TGP-TwinStrep
(Plasmid
#240251)
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Purpose(Empty Backbone) IPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tags
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240251 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepET-15b
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Modifications to backboneAltered multiple cloning site, codon-optimized thrombin cleavage site, codon-optimized tags (thermostable GFP/TGP, Twin-Strep, 10x HIS)
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Vector typeBacterial Expression
- Promoter T7
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Tags
/ Fusion Proteins
- Thrombin (C terminal on backbone)
- thermostable GFP (TGP) (C terminal on backbone)
- Twin-Strep (C terminal on backbone)
- 10x HIS (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe BL21(DE3) strain and derivatives should be used for expression.
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For compatible plasmids, please see: Addgene plasmid #159421, #159422, #159423, and #159424.
Please visit https://doi.org/10.1101/2023.12.03.569786 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15b-TGP-TwinStrep was a gift from Olga Boudker & Krishna Reddy (Addgene plasmid # 240251 ; http://n2t.net/addgene:240251 ; RRID:Addgene_240251) -
For your References section:
Evolutionary analysis reveals the origin of sodium coupling in glutamate transporters. Reddy KD, Rasool B, Akher FB, Kutlesic N, Pant S, Boudker O. bioRxiv [Preprint]. 2024 Apr 25:2023.12.03.569786. doi: 10.1101/2023.12.03.569786. 10.1101/2023.12.03.569786 PubMed 38106174