Skip to main content
Addgene
Open Menu

pSFV_Nb139-NLS-VP64 (3'UTR)_RLEloop
(Plasmid #240256)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240256 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nanobody Nb139
  • Alt name
    Nb139-VP64
  • Species
    Lama glama, Herpes simplex virus type-1
  • Mutation
    VP64 = 4*VP16[aa437-447]
  • Promoter SFV subgenomic promoter
  • Tag / Fusion Protein
    • NLS-VP64 (C terminal on insert)

Cloning Information

  • Cloning method Other

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    IDT

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Nanobody sequence was synthesized by IDT, based on protein sequence from Bethuyne et al., 2014 (https://doi.org/10.1093/nar/gku962)
VP64 was synthesized by IDT.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSFV_Nb139-NLS-VP64 (3'UTR)_RLEloop was a gift from Gregory Neely (Addgene plasmid # 240256 ; http://n2t.net/addgene:240256 ; RRID:Addgene_240256)

  • For your References section:

    A chimeric viral platform for directed evolution in mammalian cells. Cole AJ, Denes CE, Moreno CL, Hunault L, Dobson T, Hesselson D, Neely GG. Nat Commun. 2025 May 7;16(1):4250. doi: 10.1038/s41467-025-59438-2. 10.1038/s41467-025-59438-2 PubMed 40335481
Related items: