pSFV_SRF-NLS-VP64 (3'UTR)_RLEloop
(Plasmid #240258)

• Purpose: Attenuated PROTEUS vector with SRF-VP64 transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.
• Depositing Lab: Gregory Neely
• Publication: Cole et al Nat Commun. 2025 May 7;16(1):4250. doi: 10.1038/s41467-025-59438-2.

Backbone

• Vector backbone: pcDNA3.1(+)
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SRF
• Alt name: SRF-VP64
• Species: H. sapiens (human)
• Mutation: SRF[aa1-265]; VP64 = 4*VP16[aa437-447]
• Promoter: SFV subgenomic promoter
• Tag / Fusion Protein:
  • NLS-VP64 (C terminal on insert)

Cloning Information

• Cloning method: Other

Resource Information

• Supplemental Documents:
  • pSFV_SRF-NLS-VP64 (3'UTR)_RLEloop.gbk
• A portion of this plasmid was derived from a plasmid made by: IDT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SRF and VP64 sequences were synthesized by IDT.
Addgene observed 2 base pair insertion and 2 base pair deletion in the ORF. This discrepancy likely does not affect plasmid function.