pSRE_G
(Plasmid
#240260)
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PurposeCircuit-responsive (SRE promoter) expression of vesicular stomatitis virus G protein (VSVG). Used to produce envelope protein for PROTEUS VLVs during SRF-VP64 campaigns.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240260 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCMV-VSV-G
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVSV-G
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Alt nameVSVG
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SpeciesVesicular stomatitis Indiana virus
- Promoter SRE
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byJustin English, Bryan Roth (Addgene #127692), and Bob Weinberg (Addgene #8454).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SRE sequence was PCR-amplified from Addgene #127692.
VSV-G sequence backbone is Addgene #8454, PCR amplified for NEB HiFi assembly.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSRE_G was a gift from Gregory Neely (Addgene plasmid # 240260 ; http://n2t.net/addgene:240260 ; RRID:Addgene_240260) -
For your References section:
A chimeric viral platform for directed evolution in mammalian cells. Cole AJ, Denes CE, Moreno CL, Hunault L, Dobson T, Hesselson D, Neely GG. Nat Commun. 2025 May 7;16(1):4250. doi: 10.1038/s41467-025-59438-2. 10.1038/s41467-025-59438-2 PubMed 40335481