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pTRE3G_G
(Plasmid #240261)

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Item Catalog # Description Quantity Price (USD)
Plasmid 240261 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV-VSV-G
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VSV-G
  • Alt name
    VSVG
  • Species
    Vesicular stomatitis Indiana virus
  • Promoter TRE3G

Cloning Information

  • Cloning method Other

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Steven Carr, Samuel Myers (Addgene #97421), and Bob Weinberg (Addgene #8454).

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TRE3G sequence was PCR-amplified from Addgene #97421.
VSV-G sequence backbone is Addgene #8454, PCR amplified for NEB HiFi assembly.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE3G_G was a gift from Gregory Neely (Addgene plasmid # 240261 ; http://n2t.net/addgene:240261 ; RRID:Addgene_240261)

  • For your References section:

    A chimeric viral platform for directed evolution in mammalian cells. Cole AJ, Denes CE, Moreno CL, Hunault L, Dobson T, Hesselson D, Neely GG. Nat Commun. 2025 May 7;16(1):4250. doi: 10.1038/s41467-025-59438-2. 10.1038/s41467-025-59438-2 PubMed 40335481
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