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pcDNA3.1+_TRE3G_luciferase
(Plasmid #240266)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240266 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    firefly luciferase
  • Alt name
    LUC
  • Species
    Photinus pyralis
  • Promoter TRE3G

Cloning Information

  • Cloning method Other

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Steven Carr, Samuel Myers (Addgene #97421), and Jeff Holst.

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The TRE3G sequence was PCR-amplified from Addgene #97421.
The luciferase gene was PCR-amplified from plasmid pBCKS(HIV-1SDmCMVeGFP-P2A-luc2pre-IIU) described in Tiffen et al., 2010 (https://doi.org/10.1186/1476-4598-9-299)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1+_TRE3G_luciferase was a gift from Gregory Neely (Addgene plasmid # 240266 ; http://n2t.net/addgene:240266 ; RRID:Addgene_240266)

  • For your References section:

    A chimeric viral platform for directed evolution in mammalian cells. Cole AJ, Denes CE, Moreno CL, Hunault L, Dobson T, Hesselson D, Neely GG. Nat Commun. 2025 May 7;16(1):4250. doi: 10.1038/s41467-025-59438-2. 10.1038/s41467-025-59438-2 PubMed 40335481
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