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Addgene

pAAV CMV-hTau-T2A-EGFP-WPRE3-BGHpA
(Plasmid #240284)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240284 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    VectorBuilder
  • Backbone size w/o insert (bp) 4048
  • Total vector size (bp) 7327
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hMAPT
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001377265.1
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
  • Promoter CMV
  • Tag / Fusion Protein
    • T2A-EGFP (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    This plasmid is a modified version of Plasmid 140424 from the Hyman lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is an alternative to Addgene # 140424 (https://www.addgene.org/140424/) which contains a 152bp deletion in the backbone that removes the first 84bp of the ITR upstream of the CAG promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV CMV-hTau-T2A-EGFP-WPRE3-BGHpA was a gift from Bradley Hyman & Andrew Steele (Addgene plasmid # 240284)
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