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Addgene

pHnS KI;Lmnb1 Donor;3xV5 KO;Mecp2
(Plasmid #240298)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240298 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHnS
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KI gRNA for Lmnnb1
  • Alt name
    KO gRNAs for Mecp2
  • gRNA/shRNA sequence
    KI gRNA for Lmnnb1: GACGTGACAAGTTCACATAA, KO gRNAs for Mecp2: #1 GAAACCCCTGAAGTTTAAGA, #2 GCAGAGGCTTCTGGTACTGC, #3 GGTCATCATACATAGGTCCC
  • Species
    Other
  • Mutation
    NA

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHnS KI;Lmnb1 Donor;3xV5 KO;Mecp2 was a gift from Yuki Ogawa (Addgene plasmid # 240298 ; http://n2t.net/addgene:240298 ; RRID:Addgene_240298)
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