pDonr201_PARP1_del119/120
(Plasmid
#240318)
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PurposeEntry vector for Gateway with PARP1 (AA119/120 deletion)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepDonr 201
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePARP1
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SpeciesH. sapiens (human)
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MutationAA119/120 was deleted
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Entrez GenePARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDonr201_PARP1_del119/120 was a gift from Junjie Chen (Addgene plasmid # 240318 ; http://n2t.net/addgene:240318 ; RRID:Addgene_240318) -
For your References section:
DePARylation is critical for S phase progression and cell survival. Nie L, Wang C, Huang M, Liu X, Feng X, Tang M, Li S, Hang Q, Teng H, Shen X, Ma L, Gan B, Chen J. Elife. 2024 Apr 5;12:RP89303. doi: 10.7554/eLife.89303. 10.7554/eLife.89303 PubMed 38578205