pGustiv
(Plasmid #240335)

• Purpose: Expresses BK polyomavirus genotype IV capsid protein VP1 in yeast. Expression of VP1 (and a GFP reporter) is induced by maltose.
• Depositing Lab: Christopher Buck
• Publication: How to make and share vaccine-style beer (unpublished)

Backbone

• Vector backbone: pJailbreak
• Backbone manufacturer: Synthetic
• Backbone size w/o insert (bp): 1766
• Total vector size (bp): 9194
• Vector type: Yeast Expression
• Selectable markers: formaldehyde

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: BKV-IV VP1
• Species: BK polyomavirus
• Insert Size (bp): 1090
• GenBank ID: BAG84476
• Promoter: MAL32

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: Plasmidsaurus

Gene/Insert 2

• Gene/Insert name: Superfold GFP
• Insert Size (bp): 717
• GenBank ID: AEK24779
• Promoter: MAL31

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: Plasmidsaurus

Gene/Insert 3

• Gene/Insert name: Formaldehyde dehydrogenase 1
• Species: Candida maltosa
• Insert Size (bp): 1146
• GenBank ID: EMG49224
• Promoter: FDH1

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: NgoMIV (not destroyed)
• 5′ sequencing primer: Plasmidsaurus

Resource Information

• Supplemental Documents:
  • pGustiv.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA