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Addgene

pAAVS-sA-NeoR
(Plasmid #240383)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240383 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    NA
  • Vector type
    Unspecified
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NeoR
  • Insert Size (bp)
    804
  • Entrez Gene
    neoR (a.k.a. pSH111_227_223)
  • Promoter None. Driven by PPP1R12C promoter after homologous recombination

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid can be inserted into the AAV Safe Harbor site in the first intron of PPP1R12C by cleavage with cas9-gRNA. Following homologous recombination, the splice acceptor allows expression of the NeoR gene from the PPP1R12C promoter, making the cell resistant to G418.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVS-sA-NeoR was a gift from Ronald Hart (Addgene plasmid # 240383 ; http://n2t.net/addgene:240383 ; RRID:Addgene_240383)
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