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pETDuet-solo-NHis6-NocO-E252A
(Plasmid #240405)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240405 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pETDuet
  • Total vector size (bp) 6434
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nocO
  • Alt name
    NodcylC
  • Alt name
    clyC
  • Species
    Nodularia sp. 06071
  • Insert Size (bp)
    1299
  • Mutation
    M1 of native sequence not included in insert, Glutamate 252 of native sequence changed to Alanine
  • GenBank ID
    MW071172
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-solo-NHis6-NocO-E252A was a gift from Emily Balskus (Addgene plasmid # 240405 ; http://n2t.net/addgene:240405 ; RRID:Addgene_240405)

  • For your References section:

    Biochemical Studies of a Cyanobacterial Halogenase Support the Involvement of a Dimetal Cofactor. Wang ML, Glasser NR, Nair MA, Krebs C, Martin Bollinger J Jr, Balskus EP. Biochemistry. 2025 May 20;64(10):2173-2180. doi: 10.1021/acs.biochem.4c00720. Epub 2025 Apr 29. 10.1021/acs.biochem.4c00720 PubMed 40300765
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