pBluescript II NLS-MC-EGFP-tdTomato (pBET2)
(Plasmid
#240411)
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PurposeThis plasmid facilitates the assessment of MMR proficiency in human living cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240411 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepBluescript II
- Total vector size (bp) 6712
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNLS-EGFP fusion protein
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SpeciesSynthetic
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GenBank IDLC663823.1
- Promoter CMV
Cloning Information
- Cloning method Other
- 5′ sequencing primer M13 fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBluescript II NLS-MC-EGFP-tdTomato (pBET2) was a gift from Isao Kuraoka (Addgene plasmid # 240411 ; http://n2t.net/addgene:240411 ; RRID:Addgene_240411) -
For your References section:
Novel plasmids for the fluorescence-based evaluation of DNA mismatch repair in human cells. Takedachi A, Matsuishi E, Mizusaki S, Nagasawa T, Fujikane R, Hidaka M, Iwai S, Kuraoka I. Mutat Res. 2022 Jan-Jun;824:111779. doi: 10.1016/j.mrfmmm.2022.111779. Epub 2022 Apr 9. 10.1016/j.mrfmmm.2022.111779 PubMed 35472567