pHREP-RE-1-H2B
(Plasmid
#240419)
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PurposeSleeping Beauty vector for the expression of a polycistronic mRNA with shRNA resistant chicken H2B and Blasticidin resistance
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240419 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepHREP-RE-1
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Vector typeMammalian Expression
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Selectable markersHygromycin, Blasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH2B
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SpeciesG. gallus (chicken)
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Entrez GeneH2BC36 (a.k.a. 2B-VI, H2B-I, H2B-II, H2B-III, H2B-IV, H2B-VI, H2B-VII, HIST1H2B7)
- Promoter TRE
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Tags
/ Fusion Proteins
- 6xHis (C terminal on insert)
- 2xOLLAS (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer AGCTCGTTTAGTGAACCGTCAGATC
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHREP-RE-1-H2B was a gift from Lienhard Schmitz (Addgene plasmid # 240419 ; http://n2t.net/addgene:240419 ; RRID:Addgene_240419) -
For your References section:
A novel approach towards a histone replacement system in Tetrapods. Pfisterer M, Pritz A, Parry A, Schmitz ML. PLoS One 21(2): e0342014 (2026) 10.1371/journal.pone.0342014