FRB-BFP-GCN4-ppKin14
(Plasmid
#240426)
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Purpose-end oriented kinesin for rapalog induced pulling experiments
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepB80
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameppKin14Vib
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Alt nameKIN-14I
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SpeciesPhyscomitrium patens
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Mutationdeleted amino acids 1-860
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GenBank IDXM_024528909
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Tags
/ Fusion Proteins
- FRB (N terminal on insert)
- tagBFP (N terminal on insert)
- GCN4 (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.21.624725 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FRB-BFP-GCN4-ppKin14 was a gift from Anna Akhmanova (Addgene plasmid # 240426 ; http://n2t.net/addgene:240426 ; RRID:Addgene_240426) -
For your References section:
Self-assembly of pericentriolar material in interphase cells lacking centrioles. Chen F, Wu J, Iwanski MK, Jurriens D, Sandron A, Pasolli M, Puma G, Kromhout JZ, Yang C, Nijenhuis W, Kapitein LC, Berger F, Akhmanova A. Elife. 2022 Jul 5;11:e77892. doi: 10.7554/eLife.77892. 10.7554/eLife.77892 PubMed 35787744