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FRB-BFP-GCN4-ppKin14
(Plasmid #240426)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240426 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pB80
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ppKin14Vib
  • Alt name
    KIN-14I
  • Species
    Physcomitrium patens
  • Mutation
    deleted amino acids 1-850
  • GenBank ID
    XM_024528907.2
  • Tags / Fusion Proteins
    • FRB (N terminal on insert)
    • tagBFP (N terminal on insert)
    • GCN4 (N terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.21.624725 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRB-BFP-GCN4-ppKin14 was a gift from Anna Akhmanova (Addgene plasmid # 240426 ; http://n2t.net/addgene:240426 ; RRID:Addgene_240426)

  • For your References section:

    Self-assembly of pericentriolar material in interphase cells lacking centrioles. Chen F, Wu J, Iwanski MK, Jurriens D, Sandron A, Pasolli M, Puma G, Kromhout JZ, Yang C, Nijenhuis W, Kapitein LC, Berger F, Akhmanova A. Elife. 2022 Jul 5;11:e77892. doi: 10.7554/eLife.77892. 10.7554/eLife.77892 PubMed 35787744
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