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Addgene

WoFabI-pNIC-Bsa4
(Plasmid #240437)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240437 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pNIC-Bsa4
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 6166
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Enoyl-acyl carrier protein reductase
  • Alt name
    Enoyl-ACP reductase
  • Alt name
    fabI
  • Alt name
    WoFabi
  • Species
    Wolbachia endosymbiont of Brugia malayi
  • Insert Size (bp)
    811
  • GenBank ID
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His-tag (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TGTGAGCGGATAACAATTCC
  • 3′ sequencing primer AGCAGCCAACTCAGCTTCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WoFabI-pNIC-Bsa4 was a gift from Josh Beckham (Addgene plasmid # 240437 ; http://n2t.net/addgene:240437 ; RRID:Addgene_240437)
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