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pLenti Blast GRP94 N62Q V5
(Plasmid #240449)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240449 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLenti Blast
  • Backbone manufacturer
    Addgene #125133
  • Backbone size w/o insert (bp) 7600
  • Total vector size (bp) 10099
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GRP94
  • Alt name
    Endoplasmin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Mutation
    changed asparagine 62 to glutamine
  • GenBank ID
    NM_003299
  • Entrez Gene
    HSP90B1 (a.k.a. ECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 tag and second KDEL sequence (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene #82130

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti Blast GRP94 N62Q V5 was a gift from Michael Kastan (Addgene plasmid # 240449 ; http://n2t.net/addgene:240449 ; RRID:Addgene_240449)

  • For your References section:

    ATM interaction with GRP94 modulates oncogenic receptor expression and signaling and microglial activation. Burrell PE, Fleenor DE, Nicholson OM, Shao C, Kastan MB. Proc Natl Acad Sci U S A. 2025 Dec 30;122(52):e2518589122. doi: 10.1073/pnas.2518589122. Epub 2025 Dec 19. 10.1073/pnas.2518589122 PubMed 41417600
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