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hVEGF165-eGFP-hVEGF165 glycosylation sequon-His tag
(Plasmid #240484)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240484 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 5409
  • Total vector size (bp) 6303
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    glycosylated eGFP
  • Species
    Synthetic
  • Insert Size (bp)
    894
  • Tags / Fusion Proteins
    • hVEGF165 signal peptide (N terminal on insert)
    • 3 repeats of hVEGF165 glycosylation sequon (C terminal on insert)
    • 6xHis (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid was designed by our lab and cloned by Genscript.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hVEGF165-eGFP-hVEGF165 glycosylation sequon-His tag was a gift from Xi Ren (Addgene plasmid # 240484 ; http://n2t.net/addgene:240484 ; RRID:Addgene_240484)

  • For your References section:

    Glycosylation-enabled chemoselective growth factor engineering for biomaterial functionalization. Xing Y, Li Q, Otto EL, Campbell PG, Ren X. Acta Biomater. 2025 Nov;207:221-232. doi: 10.1016/j.actbio.2025.09.057. Epub 2025 Oct 1. 10.1016/j.actbio.2025.09.057 PubMed 41043672
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