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pSSBIO32_dCas9_RPA1786
(Plasmid #240489)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240489 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBHR1
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin and Gentamicin, 50 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • gRNA/shRNA sequence
    GCGCCCTGTCAGCATCATGTCGG
  • Species
    Streptococcus pyogenes
  • Mutation
    D10A, H840A
  • Entrez Gene
    cas9 (a.k.a. SPY_RS04360, SPy1046, SPy_1046)
  • Promoter Plac

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.15.638337 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSSBIO32_dCas9_RPA1786 was a gift from Rajib Saha (Addgene plasmid # 240489 ; http://n2t.net/addgene:240489 ; RRID:Addgene_240489)
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