1595-pMV-arsR-ABS
(Plasmid
#240494)
-
PurposeArsenic Biosensor
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 240494 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepMV
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameABS-arsR-ABS
-
SpeciesEscherichia coli
-
Insert Size (bp)573
-
MutationSecond ABS after arsR gene
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site SpeI (not destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
1595-pMV-arsR-ABS was a gift from Jan Roelof van der Meer (Addgene plasmid # 240494 ; http://n2t.net/addgene:240494 ; RRID:Addgene_240494) -
For your References section:
Internal arsenite bioassay calibration using multiple bioreporter cell lines. Wackwitz A, Harms H, Chatzinotas A, Breuer U, Vogne C, Van Der Meer JR. Microb Biotechnol. 2008 Mar;1(2):149-57. doi: 10.1111/j.1751-7915.2007.00011.x. 10.1111/j.1751-7915.2007.00011.x PubMed 21261832