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pCI_JUNO_H2B-RFP
(Plasmid #240498)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240498 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCI
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JUNO
  • Alt name
    Izumo1R
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Izumo1r (a.k.a. 0910001L11Rik, C86255, Folbp3, Folr4, Juno)
  • Tags / Fusion Proteins
    • V5 (C terminal on insert)
    • H2B-RFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Uknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI_JUNO_H2B-RFP was a gift from Benjamin Podbilewicz (Addgene plasmid # 240498 ; http://n2t.net/addgene:240498 ; RRID:Addgene_240498)

  • For your References section:

    A novel function for the sperm adhesion protein IZUMO1 in cell-cell fusion. Brukman NG, Nakajima KP, Valansi C, Flyak K, Li X, Higashiyama T, Podbilewicz B. J Cell Biol. 2023 Feb 6;222(2):e202207147. doi: 10.1083/jcb.202207147. Epub 2022 Nov 17. 10.1083/jcb.202207147 PubMed 36394541
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