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pCI_AFF-1-FLAG_H2B-RFP
(Plasmid #240506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240506 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AFF-1
  • Species
    C. elegans (nematode)
  • Tag / Fusion Protein
    • H2B-RFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Uknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI_AFF-1-FLAG_H2B-RFP was a gift from Benjamin Podbilewicz (Addgene plasmid # 240506 ; http://n2t.net/addgene:240506 ; RRID:Addgene_240506)

  • For your References section:

    Discovery of archaeal fusexins homologous to eukaryotic HAP2/GCS1 gamete fusion proteins. Moi D, Nishio S, Li X, Valansi C, Langleib M, Brukman NG, Flyak K, Dessimoz C, de Sanctis D, Tunyasuvunakool K, Jumper J, Grana M, Romero H, Aguilar PS, Jovine L, Podbilewicz B. Nat Commun. 2022 Jul 6;13(1):3880. doi: 10.1038/s41467-022-31564-1. 10.1038/s41467-022-31564-1 PubMed 35794124
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