pLenti-U6-epegRNA-SV40-mCherry
(Plasmid
#240538)
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PurposeLentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with mCherry. The cloning site is BsmBI.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240538 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepLenti
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersmCherry
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameepegRNA
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.06.16.599253 for the bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-U6-epegRNA-SV40-mCherry was a gift from Wensheng Wei (Addgene plasmid # 240538 ; http://n2t.net/addgene:240538 ; RRID:Addgene_240538) -
For your References section:
Prime editor-based high-throughput screening reveals functional synonymous mutations in human cells. Niu X, Tang W, Liu Y, Mo B, Yu Y, Liu Y, Wei W. Nat Biotechnol. 2025 Jun 24. doi: 10.1038/s41587-025-02710-z. 10.1038/s41587-025-02710-z PubMed 40555761