pFA6a-pmNeonGreen-kanMX6
(Plasmid
#240549)
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PurposeTag S. pombe genes at 3' end (C-terminal end of protein) with mNeonGreen, codon-optimized for S. pombe
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240549 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6a
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Vector typeYeast Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepmNeonGreen
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SpeciesSynthetic
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Insert Size (bp)708
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
mNeonGreen amino acid sequence as in Shaner, 2013 (doi:10.1038/nmeth.2413)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-pmNeonGreen-kanMX6 was a gift from Silke Hauf (Addgene plasmid # 240549 ; http://n2t.net/addgene:240549 ; RRID:Addgene_240549)