pUC18 mouse rDNA R1
(Plasmid
#240559)
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PurposeAn rDNA FISH probe contains 10.7 kb fragment that spans the 3′ end of the intergenic spacer (IGS) and the beginning of the external transcribed spacer (ETS).
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240559 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUC18
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 16379
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePartial mouse rDNA sequence
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SpeciesM. musculus (mouse)
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Insert Size (bp)13693
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GenBank IDBK000964.3
- Promoter lac
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byBrian McStay lab
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC18 mouse rDNA R1 was a gift from Brian McStay & Shan Zha (Addgene plasmid # 240559 ; http://n2t.net/addgene:240559 ; RRID:Addgene_240559)
Map uploaded by the depositor.
