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pET21a asyn(66-140) K80E/E83K
(Plasmid #240593)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240593 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET21a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    aSyn
  • Species
    H. sapiens (human)
  • Mutation
    aa 66-140 only, E83K, K80E
  • Entrez Gene
    SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a asyn(66-140) K80E/E83K was a gift from Jennifer Lee (Addgene plasmid # 240593 ; http://n2t.net/addgene:240593 ; RRID:Addgene_240593)

  • For your References section:

    Defining essential charged residues in fibril formation of a lysosomal derived N-terminal alpha-synuclein truncation. McGlinchey RP, Ramos S, Dimitriadis EK, Wilson CB, Lee JC. Nat Commun. 2025 Apr 23;16(1):3825. doi: 10.1038/s41467-025-58899-9. 10.1038/s41467-025-58899-9 PubMed 40268916
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