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Addgene

pET21a asyn(36-140)
(Plasmid #240595)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240595 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET21a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    aSyn
  • Species
    H. sapiens (human)
  • Mutation
    aa 36-140 only
  • Entrez Gene
    SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7 fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a asyn(36-140) was a gift from Jennifer Lee (Addgene plasmid # 240595 ; http://n2t.net/addgene:240595 ; RRID:Addgene_240595)

  • For your References section:

    The N terminus of alpha-synuclein dictates fibril formation. McGlinchey RP, Ni X, Shadish JA, Jiang J, Lee JC. Proc Natl Acad Sci U S A. 2021 Aug 31;118(35):e2023487118. doi: 10.1073/pnas.2023487118. 10.1073/pnas.2023487118 PubMed 34452994
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