pT7-7 asyn K80A
(Plasmid
#240599)
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PurposeOverexpress mutant alpha-Synuclein protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240599 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepT7-7
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameaSyn
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SpeciesH. sapiens (human)
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MutationK80A
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Entrez GeneSNCA (a.k.a. NACP, PARK1, PARK4, PD1)
- Promoter T7
Cloning Information
- Cloning method Other
- 5′ sequencing primer T7 fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7-7 asyn K80A was a gift from Jennifer Lee (Addgene plasmid # 240599 ; http://n2t.net/addgene:240599 ; RRID:Addgene_240599) -
For your References section:
Defining essential charged residues in fibril formation of a lysosomal derived N-terminal alpha-synuclein truncation. McGlinchey RP, Ramos S, Dimitriadis EK, Wilson CB, Lee JC. Nat Commun. 2025 Apr 23;16(1):3825. doi: 10.1038/s41467-025-58899-9. 10.1038/s41467-025-58899-9 PubMed 40268916