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Addgene

TMPRSS2-Mutant-R255A
(Plasmid #240612)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240612 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLEX307
  • Backbone size w/o insert (bp) 8416
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Type II transmembrane serine protease
  • Alt name
    TMPRSS2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1479
  • Mutation
    Arginine (R) at position 255 is replaced by alanine (A) generating a non-autocleavable form of TMPRSS2 protease
  • Entrez Gene
    TMPRSS2 (a.k.a. PRSS10)
  • Promoter EF-1a

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Alejandro Chavez & Sho Iketani (Addgene Plasmid #158457)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TMPRSS2-Mutant-R255A was a gift from Zaid Abassi (Addgene plasmid # 240612 ; http://n2t.net/addgene:240612 ; RRID:Addgene_240612)
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